5C: Pipette Calibration

Abstract

Overview of pipette calibration and its importance.

• Types of pipettes (e.g., air displacement, positive displacement)
• Common applications and significance of accurate pipetting
• Detailed explanation of gravimetric (including multi-channel pipette calibration systems) and photometric calibration methods.
• Procedures for each method.
• Comparison of methods, including advantages and limitations.
• Identification of potential sources of error in pipette calibration (e.g., environmental factors, operator technique, equipment).
• Strategies to minimize these errors.
• Methods for estimating and reporting uncertainty in calibration results.
• Standards and Guidelines: Overview of relevant national and international standards (e.g., ISO/IEC 17025, ISO 8655). Best practices and recommendations for maintaining compliance.
• Maintenance and Quality Control
• Routine maintenance procedures for pipettes.
• Quality control measures to ensure ongoing accuracy and precision
• Proper use of ergonomics for pipette calibration technicians

References: List of sources and further reading materials.